iBioSim/docs/SynBioTutorial/GeneticToggle/GeneticToggle.xml

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<?xml version="1.0" encoding="UTF-8"?>
<sbml xmlns:comp="http://www.sbml.org/sbml/level3/version1/comp/version1" xmlns:arrays="http://www.sbml.org/sbml/level3/version1/arrays/version1" xmlns:layout="http://www.sbml.org/sbml/level3/version1/layout/version1" xmlns="http://www.sbml.org/sbml/level3/version2/core" xmlns:fbc="http://www.sbml.org/sbml/level3/version1/fbc/version2" level="3" version="2" comp:required="true" fbc:required="false" arrays:required="true" layout:required="false">
<model metaid="iBioSim1" id="GeneticToggle" name="Created by libsbml flatten routine" fbc:strict="false">
<annotation>
<ModelToSBOL xmlns="http://sbolstandard.org/modeltosbol/1.0#">
<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:mts="http://sbolstandard.org/modeltosbol/1.0#">
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<rdf:Description rdf:about="#iBioSim2">
<mts:DNAComponents>
<rdf:Seq>
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<rdf:li rdf:resource="http://www.async.ece.utah.edu#comp_10_2_2014_15_7_48_611_iBioSim"/>
</rdf:Seq>
</mts:DNAComponents>
<mts:Strand>+</mts:Strand>
</rdf:Description>
</rdf:RDF>
</ModelToSBOL>
</annotation>
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<listOfCompartments>
<compartment id="Cell" spatialDimensions="3" size="1" constant="true"/>
</listOfCompartments>
<listOfSpecies>
<species metaid="iBioSim11" id="GFP" compartment="Cell" initialAmount="0" hasOnlySubstanceUnits="true" boundaryCondition="false" constant="false"/>
<species metaid="iBioSim12" id="LacI" compartment="Cell" initialAmount="60" hasOnlySubstanceUnits="true" boundaryCondition="false" constant="false"/>
<species metaid="iBioSim13" id="TetR" compartment="Cell" initialAmount="0" hasOnlySubstanceUnits="true" boundaryCondition="false" constant="false"/>
<species metaid="iBioSim14" id="IPTG" compartment="Cell" initialAmount="0" hasOnlySubstanceUnits="true" boundaryCondition="true" constant="false"/>
<species metaid="iBioSim15" id="aTc" compartment="Cell" initialAmount="0" hasOnlySubstanceUnits="true" boundaryCondition="true" constant="false"/>
<species metaid="iBioSim16" id="IPTG_LacI" compartment="Cell" initialAmount="0" hasOnlySubstanceUnits="true" boundaryCondition="false" constant="false"/>
<species metaid="iBioSim17" id="aTc_TetR" compartment="Cell" initialAmount="0" hasOnlySubstanceUnits="true" boundaryCondition="false" constant="false"/>
<species metaid="C1__iBioSim40" sboTerm="SBO:0000590" id="C1__pLac" compartment="Cell" initialAmount="2" hasOnlySubstanceUnits="true" boundaryCondition="false" constant="false"/>
<species metaid="C2__iBioSim38" sboTerm="SBO:0000590" id="C2__P0" compartment="Cell" initialAmount="2" hasOnlySubstanceUnits="true" boundaryCondition="false" constant="false"/>
</listOfSpecies>
<listOfParameters>
<parameter metaid="iBioSim3" id="kd" name="Degradation rate" value="0.0075" constant="true"/>
<parameter metaid="iBioSim4" id="kc_f" name="Forward complex formation rate" value="0.05" constant="true"/>
<parameter metaid="iBioSim5" id="kc_r" name="Reverse complex formation rate" value="1" constant="true"/>
<parameter metaid="iBioSim6" id="nc" name="Stoichiometry of binding" value="2" constant="true"/>
<parameter metaid="iBioSim7" id="kmdiff_f" name="Forward membrane diffusion rate" value="1" constant="true"/>
<parameter metaid="iBioSim8" id="kmdiff_r" name="Reverse membrane diffusion rate" value="0.01" constant="true"/>
<parameter metaid="iBioSim9" id="kecd" name="Extracellular degradation rate" value="0.005" constant="true"/>
<parameter metaid="iBioSim10" id="kecdiff" name="Extracellular diffusion rate" value="1" constant="true"/>
<parameter metaid="C1__iBioSim22" id="C1__kr_f" name="Forward repression binding rate" value="0.5" constant="true"/>
<parameter metaid="C1__iBioSim23" id="C1__kr_r" name="Reverse repression binding rate" value="1" constant="true"/>
<parameter metaid="C1__iBioSim24" id="C1__ka_f" name="Forward activation binding rate" value="0.0033" constant="true"/>
<parameter metaid="C1__iBioSim25" id="C1__ka_r" name="Reverse activation binding rate" value="1" constant="true"/>
<parameter metaid="C1__iBioSim26" id="C1__ko_f" name="Forward RNAP binding rate" value="0.033" constant="true"/>
<parameter metaid="C1__iBioSim27" id="C1__ko_r" name="Reverse RNAP binding rate" value="1" constant="true"/>
<parameter metaid="C1__iBioSim28" id="C1__kao_f" name="Forward activated RNAP binding rate" value="1" constant="true"/>
<parameter metaid="C1__iBioSim29" id="C1__kao_r" name="Reverse activated RNAP binding rate" value="1" constant="true"/>
<parameter metaid="C1__iBioSim30" id="C1__nc" name="Stoichiometry of binding" value="2" constant="true"/>
<parameter metaid="C1__iBioSim31" id="C1__nr" name="Initial RNAP count" value="30" constant="true"/>
<parameter metaid="C1__iBioSim32" id="C1__ko" name="Open complex production rate" value="0.05" constant="true"/>
<parameter metaid="C1__iBioSim33" id="C1__kb" name="Basal production rate" value="0.0001" constant="true"/>
<parameter metaid="C1__iBioSim34" id="C1__ng" name="Initial promoter count" value="2" constant="true"/>
<parameter metaid="C1__iBioSim35" id="C1__np" name="Stoichiometry of production" value="10" constant="true"/>
<parameter metaid="C1__iBioSim36" id="C1__ka" name="Activated production rate" value="0.25" constant="true"/>
<parameter metaid="C2__iBioSim21" id="C2__kr_f" name="Forward repression binding rate" value="0.5" constant="true"/>
<parameter metaid="C2__iBioSim22" id="C2__kr_r" name="Reverse repression binding rate" value="1" constant="true"/>
<parameter metaid="C2__iBioSim23" id="C2__ka_f" name="Forward activation binding rate" value="0.0033" constant="true"/>
<parameter metaid="C2__iBioSim24" id="C2__ka_r" name="Reverse activation binding rate" value="1" constant="true"/>
<parameter metaid="C2__iBioSim25" id="C2__ko_f" name="Forward RNAP binding rate" value="0.033" constant="true"/>
<parameter metaid="C2__iBioSim26" id="C2__ko_r" name="Reverse RNAP binding rate" value="1" constant="true"/>
<parameter metaid="C2__iBioSim27" id="C2__kao_f" name="Forward activated RNAP binding rate" value="1" constant="true"/>
<parameter metaid="C2__iBioSim28" id="C2__kao_r" name="Reverse activated RNAP binding rate" value="1" constant="true"/>
<parameter metaid="C2__iBioSim29" id="C2__nc" name="Stoichiometry of binding" value="2" constant="true"/>
<parameter metaid="C2__iBioSim30" id="C2__nr" name="Initial RNAP count" value="30" constant="true"/>
<parameter metaid="C2__iBioSim31" id="C2__ko" name="Open complex production rate" value="0.05" constant="true"/>
<parameter metaid="C2__iBioSim32" id="C2__kb" name="Basal production rate" value="0.0001" constant="true"/>
<parameter metaid="C2__iBioSim33" id="C2__ng" name="Initial promoter count" value="2" constant="true"/>
<parameter metaid="C2__iBioSim34" id="C2__np" name="Stoichiometry of production" value="10" constant="true"/>
<parameter metaid="C2__iBioSim35" id="C2__ka" name="Activated production rate" value="0.25" constant="true"/>
</listOfParameters>
<listOfReactions>
<reaction metaid="iBioSim18" sboTerm="SBO:0000179" id="Degradation_GFP" reversible="false" compartment="Cell">
<listOfReactants>
<speciesReference species="GFP" stoichiometry="1" constant="true"/>
</listOfReactants>
<kineticLaw>
<math xmlns="http://www.w3.org/1998/Math/MathML">
<apply>
<times/>
<ci> kd </ci>
<ci> GFP </ci>
</apply>
</math>
</kineticLaw>
</reaction>
<reaction metaid="iBioSim19" sboTerm="SBO:0000179" id="Degradation_TetR" reversible="false" compartment="Cell">
<listOfReactants>
<speciesReference species="TetR" stoichiometry="1" constant="true"/>
</listOfReactants>
<kineticLaw>
<math xmlns="http://www.w3.org/1998/Math/MathML">
<apply>
<times/>
<ci> kd </ci>
<ci> TetR </ci>
</apply>
</math>
</kineticLaw>
</reaction>
<reaction metaid="iBioSim20" sboTerm="SBO:0000179" id="Degradation_IPTG_LacI" reversible="false" compartment="Cell">
<listOfReactants>
<speciesReference species="IPTG_LacI" stoichiometry="1" constant="true"/>
</listOfReactants>
<kineticLaw>
<math xmlns="http://www.w3.org/1998/Math/MathML">
<apply>
<times/>
<ci> kd </ci>
<ci> IPTG_LacI </ci>
</apply>
</math>
</kineticLaw>
</reaction>
<reaction metaid="iBioSim21" sboTerm="SBO:0000179" id="Degradation_aTc_TetR" reversible="false" compartment="Cell">
<listOfReactants>
<speciesReference species="aTc_TetR" stoichiometry="1" constant="true"/>
</listOfReactants>
<kineticLaw>
<math xmlns="http://www.w3.org/1998/Math/MathML">
<apply>
<times/>
<ci> kd </ci>
<ci> aTc_TetR </ci>
</apply>
</math>
</kineticLaw>
</reaction>
<reaction metaid="iBioSim22" sboTerm="SBO:0000177" id="Complex_IPTG_LacI" reversible="true" compartment="Cell">
<listOfReactants>
<speciesReference species="IPTG" stoichiometry="1" constant="true"/>
<speciesReference species="LacI" stoichiometry="1" constant="true"/>
</listOfReactants>
<listOfProducts>
<speciesReference species="IPTG_LacI" stoichiometry="1" constant="true"/>
</listOfProducts>
<kineticLaw>
<math xmlns="http://www.w3.org/1998/Math/MathML">
<apply>
<minus/>
<apply>
<times/>
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<ci> kc_f </ci>
<apply>
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<power/>
<ci> IPTG </ci>
<ci> nc_IPTG </ci>
</apply>
<apply>
<power/>
<ci> LacI </ci>
<ci> nc_LacI </ci>
</apply>
</apply>
<apply>
<times/>
<ci> kc_r </ci>
<ci> IPTG_LacI </ci>
</apply>
</apply>
</math>
<listOfLocalParameters>
<localParameter id="nc_LacI" value="1"/>
<localParameter id="nc_IPTG" value="1"/>
</listOfLocalParameters>
</kineticLaw>
</reaction>
<reaction metaid="iBioSim23" sboTerm="SBO:0000177" id="Complex_aTc_TetR" reversible="true" compartment="Cell">
<listOfReactants>
<speciesReference species="TetR" stoichiometry="1" constant="true"/>
<speciesReference species="aTc" stoichiometry="1" constant="true"/>
</listOfReactants>
<listOfProducts>
<speciesReference species="aTc_TetR" stoichiometry="1" constant="true"/>
</listOfProducts>
<kineticLaw>
<math xmlns="http://www.w3.org/1998/Math/MathML">
<apply>
<minus/>
<apply>
<times/>
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<ci> kc_f </ci>
<apply>
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<power/>
<ci> TetR </ci>
<ci> nc_TetR </ci>
</apply>
<apply>
<power/>
<ci> aTc </ci>
<ci> nc_aTc </ci>
</apply>
</apply>
<apply>
<times/>
<ci> kc_r </ci>
<ci> aTc_TetR </ci>
</apply>
</apply>
</math>
<listOfLocalParameters>
<localParameter id="nc_aTc" value="1"/>
<localParameter id="nc_TetR" value="1"/>
</listOfLocalParameters>
</kineticLaw>
</reaction>
<reaction metaid="iBioSim24" sboTerm="SBO:0000179" id="Degradation_LacI" reversible="false" compartment="Cell">
<listOfReactants>
<speciesReference species="LacI" stoichiometry="1" constant="true"/>
</listOfReactants>
<kineticLaw>
<math xmlns="http://www.w3.org/1998/Math/MathML">
<apply>
<times/>
<ci> kd </ci>
<ci> LacI </ci>
</apply>
</math>
</kineticLaw>
</reaction>
<reaction metaid="C1__iBioSim41" sboTerm="SBO:0000589" id="C1__Production_pLac" reversible="false" compartment="Cell">
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<listOfProducts>
<speciesReference species="TetR" stoichiometry="10" constant="true"/>
<speciesReference species="GFP" stoichiometry="10" constant="true"/>
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</listOfProducts>
<listOfModifiers>
<modifierSpeciesReference sboTerm="SBO:0000598" species="C1__pLac"/>
<modifierSpeciesReference sboTerm="SBO:0000020" species="LacI"/>
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</listOfModifiers>
<kineticLaw>
<math xmlns="http://www.w3.org/1998/Math/MathML">
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<apply>
<divide/>
<apply>
<times/>
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<ci> C1__pLac </ci>
<ci> C1__ko </ci>
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<apply>
<divide/>
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<ci> C1__ko_f </ci>
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<ci> C1__ko_r </ci>
</apply>
<ci> C1__nr </ci>
</apply>
<apply>
<plus/>
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<cn type="integer"> 1 </cn>
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<apply>
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<times/>
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<apply>
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<divide/>
<ci> C1__ko_f </ci>
<ci> C1__ko_r </ci>
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</apply>
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<ci> C1__nr </ci>
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</apply>
<apply>
<power/>
<apply>
<times/>
<apply>
<divide/>
<ci> C1__kr_f </ci>
<ci> C1__kr_r </ci>
</apply>
<ci> LacI </ci>
</apply>
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<ci> C1__nc </ci>
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</apply>
</apply>
</apply>
</math>
</kineticLaw>
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</reaction>
<reaction metaid="C2__iBioSim39" sboTerm="SBO:0000589" id="C2__Production_P0" reversible="false" compartment="Cell">
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<listOfProducts>
<speciesReference species="LacI" stoichiometry="10" constant="true"/>
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</listOfProducts>
<listOfModifiers>
<modifierSpeciesReference sboTerm="SBO:0000598" species="C2__P0"/>
<modifierSpeciesReference sboTerm="SBO:0000020" species="TetR"/>
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</listOfModifiers>
<kineticLaw>
<math xmlns="http://www.w3.org/1998/Math/MathML">
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<apply>
<divide/>
<apply>
<times/>
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<ci> C2__P0 </ci>
<ci> C2__ko </ci>
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<apply>
<divide/>
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<ci> C2__ko_f </ci>
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<ci> C2__ko_r </ci>
</apply>
<ci> C2__nr </ci>
</apply>
<apply>
<plus/>
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<cn type="integer"> 1 </cn>
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<apply>
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<times/>
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<apply>
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<divide/>
<ci> C2__ko_f </ci>
<ci> C2__ko_r </ci>
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</apply>
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<ci> C2__nr </ci>
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</apply>
<apply>
<power/>
<apply>
<times/>
<apply>
<divide/>
<ci> C2__kr_f </ci>
<ci> C2__kr_r </ci>
</apply>
<ci> TetR </ci>
</apply>
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<ci> C2__nc </ci>
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</apply>
</apply>
</apply>
</math>
</kineticLaw>
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</reaction>
</listOfReactions>
<listOfEvents>
<event metaid="iBioSim25" id="IPTG_High" useValuesFromTriggerTime="false">
<trigger initialValue="false" persistent="false">
<math xmlns="http://www.w3.org/1998/Math/MathML">
<true/>
</math>
</trigger>
<delay>
<math xmlns="http://www.w3.org/1998/Math/MathML">
<cn type="integer"> 2000 </cn>
</math>
</delay>
<listOfEventAssignments>
<eventAssignment variable="IPTG">
<math xmlns="http://www.w3.org/1998/Math/MathML">
<cn type="integer"> 60 </cn>
</math>
</eventAssignment>
</listOfEventAssignments>
</event>
<event metaid="iBioSim26" id="IPTG_Low" useValuesFromTriggerTime="false">
<trigger initialValue="false" persistent="false">
<math xmlns="http://www.w3.org/1998/Math/MathML">
<true/>
</math>
</trigger>
<delay>
<math xmlns="http://www.w3.org/1998/Math/MathML">
<cn type="integer"> 4000 </cn>
</math>
</delay>
<listOfEventAssignments>
<eventAssignment variable="IPTG">
<math xmlns="http://www.w3.org/1998/Math/MathML">
<cn type="integer"> 0 </cn>
</math>
</eventAssignment>
</listOfEventAssignments>
</event>
<event metaid="iBioSim27" id="aTc_High" useValuesFromTriggerTime="false">
<trigger initialValue="false" persistent="false">
<math xmlns="http://www.w3.org/1998/Math/MathML">
<true/>
</math>
</trigger>
<delay>
<math xmlns="http://www.w3.org/1998/Math/MathML">
<cn type="integer"> 6000 </cn>
</math>
</delay>
<listOfEventAssignments>
<eventAssignment variable="aTc">
<math xmlns="http://www.w3.org/1998/Math/MathML">
<cn type="integer"> 60 </cn>
</math>
</eventAssignment>
</listOfEventAssignments>
</event>
<event metaid="iBioSim28" id="aTc_Low" useValuesFromTriggerTime="false">
<trigger initialValue="false" persistent="false">
<math xmlns="http://www.w3.org/1998/Math/MathML">
<true/>
</math>
</trigger>
<delay>
<math xmlns="http://www.w3.org/1998/Math/MathML">
<cn type="integer"> 8000 </cn>
</math>
</delay>
<listOfEventAssignments>
<eventAssignment variable="aTc">
<math xmlns="http://www.w3.org/1998/Math/MathML">
<cn type="integer"> 0 </cn>
</math>
</eventAssignment>
</listOfEventAssignments>
</event>
</listOfEvents>
<layout:listOfLayouts xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:layout="http://www.sbml.org/sbml/level3/version1/layout/version1">
<layout:layout layout:id="iBioSim">
<layout:dimensions layout:width="1070" layout:height="425"/>
<layout:listOfCompartmentGlyphs>
<layout:compartmentGlyph layout:id="C1__Glyph__Cell" layout:compartment="Cell">
<layout:boundingBox>
<layout:position layout:x="0" layout:y="0"/>
<layout:dimensions layout:width="1070" layout:height="425"/>
</layout:boundingBox>
</layout:compartmentGlyph>
</layout:listOfCompartmentGlyphs>
<layout:listOfSpeciesGlyphs>
<layout:speciesGlyph layout:id="C1__Glyph__LacI" layout:species="LacI">
<layout:boundingBox>
<layout:position layout:x="98" layout:y="66"/>
<layout:dimensions layout:width="100" layout:height="30"/>
</layout:boundingBox>
</layout:speciesGlyph>
<layout:speciesGlyph layout:id="C1__Glyph__TetR" layout:species="TetR">
<layout:boundingBox>
<layout:position layout:x="90" layout:y="290"/>
<layout:dimensions layout:width="100" layout:height="30"/>
</layout:boundingBox>
</layout:speciesGlyph>
<layout:speciesGlyph layout:id="C1__Glyph__GFP" layout:species="GFP">
<layout:boundingBox>
<layout:position layout:x="290" layout:y="290"/>
<layout:dimensions layout:width="100" layout:height="30"/>
</layout:boundingBox>
</layout:speciesGlyph>
<layout:speciesGlyph layout:id="C1__Glyph__pLac" layout:species="C1__pLac">
<layout:boundingBox>
<layout:position layout:x="100" layout:y="170"/>
<layout:dimensions layout:width="100" layout:height="30"/>
</layout:boundingBox>
</layout:speciesGlyph>
</layout:listOfSpeciesGlyphs>
<layout:listOfTextGlyphs>
<layout:textGlyph layout:id="C1__TextGlyph__Cell" layout:text="Cell" layout:graphicalObject="C1__Glyph__Cell">
<layout:boundingBox>
<layout:position layout:x="0" layout:y="0"/>
<layout:dimensions layout:width="1070" layout:height="425"/>
</layout:boundingBox>
</layout:textGlyph>
<layout:textGlyph layout:id="C1__TextGlyph__LacI" layout:text="LacI" layout:graphicalObject="C1__Glyph__LacI">
<layout:boundingBox>
<layout:position layout:x="98" layout:y="66"/>
<layout:dimensions layout:width="100" layout:height="30"/>
</layout:boundingBox>
</layout:textGlyph>
<layout:textGlyph layout:id="C1__TextGlyph__TetR" layout:text="TetR" layout:graphicalObject="C1__Glyph__TetR">
<layout:boundingBox>
<layout:position layout:x="90" layout:y="290"/>
<layout:dimensions layout:width="100" layout:height="30"/>
</layout:boundingBox>
</layout:textGlyph>
<layout:textGlyph layout:id="C1__TextGlyph__GFP" layout:text="GFP" layout:graphicalObject="C1__Glyph__GFP">
<layout:boundingBox>
<layout:position layout:x="290" layout:y="290"/>
<layout:dimensions layout:width="100" layout:height="30"/>
</layout:boundingBox>
</layout:textGlyph>
<layout:textGlyph layout:id="C1__TextGlyph__pLac" layout:text="pLac" layout:graphicalObject="C1__Glyph__pLac">
<layout:boundingBox>
<layout:position layout:x="100" layout:y="170"/>
<layout:dimensions layout:width="100" layout:height="30"/>
</layout:boundingBox>
</layout:textGlyph>
</layout:listOfTextGlyphs>
</layout:layout>
<layout:layout layout:id="C2__iBioSim">
<layout:dimensions layout:width="1070" layout:height="425"/>
<layout:listOfCompartmentGlyphs>
<layout:compartmentGlyph layout:id="C2__Glyph__Cell" layout:compartment="Cell">
<layout:boundingBox>
<layout:position layout:x="0" layout:y="0"/>
<layout:dimensions layout:width="1070" layout:height="425"/>
</layout:boundingBox>
</layout:compartmentGlyph>
</layout:listOfCompartmentGlyphs>
<layout:listOfSpeciesGlyphs>
<layout:speciesGlyph layout:id="C2__Glyph__TetR" layout:species="TetR">
<layout:boundingBox>
<layout:position layout:x="123" layout:y="65"/>
<layout:dimensions layout:width="100" layout:height="30"/>
</layout:boundingBox>
</layout:speciesGlyph>
<layout:speciesGlyph layout:id="C2__Glyph__LacI" layout:species="LacI">
<layout:boundingBox>
<layout:position layout:x="138" layout:y="216"/>
<layout:dimensions layout:width="100" layout:height="30"/>
</layout:boundingBox>
</layout:speciesGlyph>
</layout:listOfSpeciesGlyphs>
<layout:listOfTextGlyphs>
<layout:textGlyph layout:id="C2__TextGlyph__Cell" layout:text="Cell" layout:graphicalObject="C2__Glyph__Cell">
<layout:boundingBox>
<layout:position layout:x="0" layout:y="0"/>
<layout:dimensions layout:width="1070" layout:height="425"/>
</layout:boundingBox>
</layout:textGlyph>
<layout:textGlyph layout:id="C2__TextGlyph__TetR" layout:text="TetR" layout:graphicalObject="C2__Glyph__TetR">
<layout:boundingBox>
<layout:position layout:x="123" layout:y="65"/>
<layout:dimensions layout:width="100" layout:height="30"/>
</layout:boundingBox>
</layout:textGlyph>
<layout:textGlyph layout:id="C2__TextGlyph__LacI" layout:text="LacI" layout:graphicalObject="C2__Glyph__LacI">
<layout:boundingBox>
<layout:position layout:x="138" layout:y="216"/>
<layout:dimensions layout:width="100" layout:height="30"/>
</layout:boundingBox>
</layout:textGlyph>
</layout:listOfTextGlyphs>
</layout:layout>
</layout:listOfLayouts>
</model>
</sbml>